The Transposon Registry

Displaying 281 - 300 of 2071
Tn5405
  • Antibiotic Resistance
  • Kanamycin
  • Neomycin
  • Streptomycin
Characteristics
Tn5405 is a 12-kb composite transposon found as a part of another transposon Tn5404. It contains two aminoglycosides resistance genes, aphA-3 and aadE, which are flanked by inverted repeats of IS1182.
References

Derbise A, de Cespedes G & el Solh N (1997) Nucleotide sequence of the Staphylococcus aureus transposon, Tn5405, carrying aminoglycosides resistance genes. Journal of basic microbiology 37: 379-384. DOI: 10.1002/jobm.3620370511

Accession number
Tn5406
  • Antibiotic Resistance
  • Streptogramin A
Characteristics
Tn5406 is a 5.5-kb transposon isolated from a clinical isolate of Staphylococcus aureus (BM3327). It carries transposase genes (tnpA,B and C) and a variant of vgaA, which encodes a putative ABC protein conferring streptogramin A resistance.
References

Haroche, J., Allignet, J., & El Solh, N. (2002). Tn5406, a New Staphylococcal Transposon Conferring Resistance to Streptogramin A and Related Compounds Including Dalfopristin. Antimicrobial Agents and Chemotherapy, 46(8), 2337–2343. DOI: 10.1128/AAC.46.8.2337-2343.2002

Accession number
Tn5422
  • Heavy Metal Resistance
  • Cadmium Resistance
Characteristics
Tn5422 is a 6.5-kb transposon, closely related to Tn917, which is found on plasmids from Listeria monocytogenes. It contains cadmium resistance genes (cadA and cadC), transposition genes (tnpA and tnpR) and a putative recombination site (res).
References

Lebrun, M., Audurier, A., & Cossart, P. (1994). Plasmid-borne cadmium resistance genes in Listeria monocytogenes are present on Tn5422, a novel transposon closely related to Tn917. Journal of Bacteriology, 176(10), 3049–3061. DOI: 10.1128/jb.176.10.3049-3061.1994

Accession number
Tn5431
  • Antibiotic Resistance
  • Ampicillin
  • Tetracycline
Characteristics
Tn5431 is a 16-kb transposon, isolated from plasmid pRK100 from Escherichia coli. It was demonstrated that Tn5431 arose by transposition of Tn3 (containing bla, mutated tnpA and tnpR genes) into Tn1721 (containing tet, tnpA, tnpR and orfI genes).
References

Zgur-Bertok D, Ambrozic J & Grabnar M (1996) Tn5431 arose by transposition of Tn3 into Tn1721. Canadian journal of microbiology 42: 1274-1276. DOI: 10.1139/m96-166

Accession number
Tn5432
  • Antibiotic Resistance
  • Clindamycin
  • Erythromycin
  • Streptogramin B
Characteristics
Tn5432 is a 4.5-kb composite transposon, identified on plasmid pTP10 from the clinical isolate Corynebacterium xerosis. It contains an erythromycin resistance gene erm(X) (conferring macrolide-lincosamide-streptogramin B resistance), which is flanked by two IS1249 elements.
References

Tauch A, Kassing F, Kalinowski J & Puhler A (1995) The Corynebacterium xerosis composite transposon Tn5432 consists of two identical insertion sequences, designated IS1249, flanking the erythromycin resistance gene ermCX. Plasmid 34: 119-131. DOI: 10.1006/plas.1995.9995

Accession number
Tn5466
  • Antibiotic Resistance
  • Erythromycin
  • Streptomycin
Characteristics
Tn5466 is an 8.8-kb composite transposon, found on plasmid pDML508 from the multidrug-resistant strain Enterococcus hirae S185. It contains an inverted repeat of IS1216 elements, which is flanking methylase-encoding ermAM and adenylyl-transferase-encoding aadE genes, conferring erythromycin and streptomycin resistance, respectively.
References

Raze, D., et al. (1998). The Gene Encoding the Low-Affinity Penicillin-Binding Protein 3r in Enterococcus hirae S185R Is Borne on a Plasmid Carrying Other Antibiotic Resistance Determinants. Antimicrobial Agents and Chemotherapy, 42(3), 534–539. PMID: 9517928

Accession number
Tn5467
Characteristics
Tn5467 is a 3.5-kb Tn21-like transposon, found on plasmid pTF-FC2 from Thiobacillus ferrooxidans. It contains genes encoding thioredoxin, MerR regulatory protein and inactive transposase.
References

Clennel, A. M., Johnston, B., & Rawlings, D. E. (1995). Structure and function of Tn5467, a Tn21-like transposon located on the Thiobacillus ferrooxidans broad-host-range plasmid pTF-FC2. Applied and Environmental Microbiology, 61(12), 4223–4229. PMID: 8534089

Accession number
Tn5468
Characteristics
Tn5468 is an 8-kb Tn7-like transposon, found in Thiobacillus ferrooxidans ATCC 33020. It contains transposition genes tnsA, tnsB, tnsC, and tnsD.
References

Oppon, J. C., et al. (1998). A Tn7-Like Transposon Is Present in the glmUS Region of the Obligately Chemoautolithotrophic Bacterium Thiobacillus ferrooxidans. Journal of Bacteriology, 180(11), 3007–3012. PMID: 9603897

Accession number
Tn5469
Characteristics
Tn5469 is a 4.9-kb transposon, isolated from Fremyella diplosiphon. It contains three slightly overlapping potential ORFs, encoding TnpA and two proteins with unknown function.
References

Kahn, K., & Schaefer, M. R. (1995). Characterization of transposon Tn5469 from the cyanobacterium Fremyella diplosiphon. Journal of Bacteriology, 177(24), 7026–7032. DOI: 10.1128/jb.177.24.7026-7032.1995

Accession number
Tn5481
Characteristics
Tn5481 is a 5-kb transposon isolated from Lactococcus lactis N8. It contains orfA, orfB orfC and orfD (encoding hypothetical proteins), and nisG gene (nisin immunity).
References

Immonen T, Wahlstrom G, Takala T & Saris PE (1998) Evidence for a mosaic structure of the Tn5481 in Lactococcus lactis N8. DNA sequence : the journal of DNA sequencing and mapping 9: 245-261. DOI: 10.3109/10425179809008465

Accession number
Tn5491
  • Antibiotic Resistance
  • Tetracycline
Characteristics
Tn5491 is constructed based on Tn1721 (containing tetA and tetR genes) by adding a universal M13 primer binding site (UP) for DNA sequencing to the left end of Tn1721.
References

Fischer, J., et al. (1996). "The use of an improved transposon mutagenesis system for DNA sequencing leads to the characterization of a new insertion sequence of Streptomyces lividans 66." Gene 180(1-2): 81-89. DOI: 10.1016/S0378-1119(96)00409-X

Tn5492
  • Antibiotic Resistance
  • Thiostrepton
Characteristics
Tn5492 is located on plasmid pJOE2129 and constructed based on IS1373, identified from Streptomyces lividans. Thiostrepton resistance gene (tsr) was inserted between the C-terminal end of the putative transposase gene insA and the next IR of IS1373.
References

Volff, J. N., & Altenbuchner, J. (1997). High-frequency transposition of IS1373, the insertion sequence delimiting the amplifiable element AUD2 of Streptomyces lividans. Journal of Bacteriology, 179(17), 5639–5642. DOI: 10.1128/jb.179.17.5639-5642.1997

Tn5493
  • Antibiotic Resistance
  • Thiostrepton
Characteristics
Tn5493 is a mini-Tn5 derivative transposon, which is a part of transposon delivery vector pJOE2577, constructed as a tool for genetic and molecular analysis of the antibiotic-producing streptomycetes. It contains thiostrepton resistance gene (tsr), tnpA of IS50 elements.
References

Volff, J. N. and J. Altenbuchner (1997). "High frequency transposition of the Tn5 derivative Tn5493 in Streptomyces lividans." Gene 194(1): 81-86. DOI: 10.1016/S0378-1119(97)00163-7

Tn5495
  • Antibiotic Resistance
  • Kanamycin
Characteristics
Tn5495 is a mini-transposon derived from Tn5, located on plasmid pCro2a. It contains kanamycin resistance genes.
References

Onaca, C. et al. (2007). Degradation of Alkyl Methyl Ketones by Pseudomonas veronii MEK700 . Journal of Bacteriology, 189(10), 3759–3767. DOI: 10.1128/JB.01279-06

Tn5501
Characteristics
Tn5501 is a member of Tn3 family with a size of 5.6 kb, which was found on plasmid pGH1 of Pseudomonas putida strain H. It contains tnpA and tnpR, encoding transposase and resolvase, respectively.
References

Lauf, U., et al. (1998). The transposable elements resident on the plasmids of Pseudomonas putida strain H, Tn5501 and Tn5502, are cryptic transposons of the Tn3 family. Molecular and General Genetics MGG 259(6): 674-678. DOI: 10.1007/s004380050862

Accession number
Tn5502
Characteristics
Tn5502 is a 9 kb composite transposon containing Tn5501 and a 3.8 kb DNA fragment, isolated from Pseudomonas putida strain H. The DNA fragment contained orf109, orf545 and orf114.
References

Lauf, U., et al. (1998). The transposable elements resident on the plasmids of Pseudomonas putida strain H, Tn5501 and Tn5502, are cryptic transposons of the Tn3 family. Molecular and General Genetics MGG 259(6): 674-678. DOI: 10.1007/s004380050862

Accession number
Tn5506
  • Antibiotic Resistance
  • Erythromycin
Characteristics
Tn5506 is a 39-kb composite transposon, found on plasmid pHKK702 of Enterococcus faecium. It is flanked by IS1216V1 and IS1216V2 elements.
References

Heaton, M. P., et al. (1996). "Mobilization of vancomycin resistance by transposon-mediated fusion of a VanA plasmid with an Enterococcus faecium sex pheromone-response plasmid." Gene 171(1): 9-17. DOI: 10.1016/0378-1119(96)00022-4

Accession number
Tn5520
Characteristics
Tn5520 is a 4.7-kb transposon found in Bacteroides fragilis. It contains bipH and bmpH genes, encoding proteins involving in transposition and mobility, respectively.
References

Gayatri, V., et al. (2006). "Bacteroides fragilis mobilizable transposon Tn5520 requires a 71 base pair origin of transfer sequence and a single mobilization protein for relaxosome formation during conjugation." Mol Microbiol 59(1): 288-300. DOI: 10.1111/j.1365-2958.2005.04934.x

Accession number
Tn5530
  • Efflux functions
  • Metabolism
  • 2,4-dichlorophenoxyacetate dissimilatory
  • Malonate dissimilatory
Characteristics
Tn5530 is a 36-kb composite transposon-like structure, found on plasmid pIJB1 of Burkholderia cepacia strain 2a. It is flanked by IS1071::IS1471 elements and contains 29 complete ORFs, encoding proteins involving in the catabolism of 2,4-dichlorophenoxyacetate (2,4-D) and malonate, chloride-channel protein (expulsion of chloride ions), leucine-responsive regulatory factor (lrp) transposase and proline dehydrogenase (putA).
References

Poh, R. P. C., et al. (2002). "Complete characterisation of Tn5530 from Burkholderia cepacia strain 2a (pIJB1) and studies of 2,4-dichlorophenoxyacetate uptake by the organism." Plasmid 48(1): 1-12. DOI: 10.1016/S0147-619X(02)00018-5

Accession number
Tn5531
  • Antibiotic Resistance
  • Kanamycin
Characteristics
Tn5531 is a 4.5-kb composite transposon, found on plasmid pCGL0040, which can be used for insertional mutagenesis of Corynebacterium glutamicum. It contains aph3 gene conferring kanamycin resistance, flanked by two IS1207 elements.
References

Bonamy, C., et al. (2003). "The mobile element IS1207 of Brevibacterium lactofermentum ATCC21086: isolation and use in the construction of Tn5531, a versatile transposon for insertional mutagenesis of Corynebacterium glutamicum." Journal of Biotechnology 104(1): 301-309. DOI: 10.1016/S0168-1656(03)00150-0

Accession number

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