The Transposon Registry

Tn6162 is a 26-kb Tn1403-like transposon, found in genomic island 1 of Pseudomonas aeruginosa. It contained Tn1403 transposition module, ISpA7, mercury resistance operon (merEDAPTR), and class 1 integron [containing sul1 gene in 3'CS, two gene cassettes (gcuD and aadA6), and integron integrase gene].

Tn6164 is a 106-kb transposon, found in Clostridium difficile strain M120 (PCR ribotype 078 strain). The 3’ end of Tn6164 is homologous to Tn1806 of Streptococcus pneumoniae [conferring erythromycin resistance], which appears to be the backbone into which several other elements have been inserted. The first 7.3 kb on the 5’ end of the insert contained 2 putative modification DNA methylases and a putative endonuclease. This sequence is followed by a 39.5-kb complete prophage, similar to a Thermoanaerobacter sp. prophage. The next 4.5 kb stretch is almost identical to part of the Enterococcus faecalis plasmid pEF418 containing, a putative methyltransferase and a putative spectinomycin adenyltransferase (ant(9)Ia). Finally, an insertion of approximately 4.5 kb is homologous to pathogenicity island of Campylobacter fetus subsp fetus, which contained putative tet(44) and ant(6)-Ib genes.