The Transposon Registry

Tn6652 is a 10.2-kb composite transposon, found on plasmid pUR-EC07 in Escherichia coli strain EC07. It contains two IS26 elements, flanking ermBL-ermB operon. It shared one of the IS26 with another composite transposon, Tn6652.

Tn6656 is a 3358 bp composite transposon consisting of two copies of ISAba1 (inversely oriented) flanking a 907 bp fragment of DNA that harbours blaOXA-235 (831 bp) with an additional 91 bp of the left end of ISAba1 in inverse orientation found at the right end. Thus, Tn6252 (ISAba1-blaOXA-235-ISAba1; 3267 bp) is wholly contained within Tn6656. Tn6656 is found in three copies in the chromosomes of A. baumannii 09A16CRGN003B and 09A16CRGN014. These two chromosomes also harbour a single copy of Tn6252.

Tn6658 is a 3940 bp composite transposon consisting of two copies of ISAba1 (inversely oriented) flanking a 1580 bp of DNA that contains a CRP gene (cAMP-activated global transcriptional regulator)(666 bp) and a OsmC family protein gene (osmotically inducible stressed-induced protein)(423 bp). The Tn6658 sequence is found in two copies in the chromosomes of A. baumannii 09A16CRGN014 and 09A16CRGN003B, six copies in 11A1213CRGN008, seven copies in 11A1213CRGN055 and 11A1314CRGN088, and eight copies in 11A14CRGN003. One copy in each chromosome, Tn6658alpha, can be considered to be the progenitor of the transposon as each of the copies of ISAba1 is flanked by their own unique 9 bp direct repeats indicating separate insertion events in this chromosomal region. Tn6658alpha can then be postulated to have transposed as a unit, Tn6658, to a different regions of the chromosome where it inserted by transposition as it is flanked by identical 9 bp direct repeats.

ICE6660 is a novel integrative and conjugative element (ICE) of the clc family, found in clincal strain of Pseudomonas aeruginosa. It is 112205 bp-long structure inserted into a tRNAGly gene (PAO1 locus PA2583). It carried various antibitoic resistance genes, e.g. blaNDM-1, blaPME-1 and rmtD3. It has two variable regions: cargo1 (~25 kb), between the P4 integrase and relaxase genes, and cargo2 (~30 kb) within the DNA methyltransferase gene.