Tn registry

Displaying 901 - 920 of 950
Tn6638
Lang Yang
Tn6639
Lang Yang
Tn6640
Renata Galetti
Tn6644
Dr Marco Maria D'Andrea
University of Siena
  • Antibiotic Resistance
  • Azithromycin
  • Chloramphenicol
  • Lincomycin
  • Oxazolidinone
  • Streptogramin A
  • Tiamulin
Characteristics
Tn6644 is a 5.1-kb composite transposon composed by two ISEnfa5 arranged in the same orientation flanking the cfr multiresistance gene. This structure has been found in several microbial species, either located in the chromosome or on plasmids. Its mobilization by transposition could be suggested observing the different target site duplications of 3 bp found in distinct genetic environments. A part of Tn6644 comprising a copy of ISEnfa5, the cfr gene and the sequences between cfr and two ISEnfa5 elements, is able to looping-out giving circular intermediates. The passenger gene cfr is a rRNA methyltransferase that acts on a conserved 23S rRNA residue, conferring resistance to an array of ribosomal antibiotics, including phenicols, lincosamides, pleuromutilins, streptogramins A, 16-member ring macrolides and oxazolidinones.
References

D'Andrea, M.M. et al. 2018. Direct submission.

Wang Y, Li D, Song L, et al. First report of the multiresistance gene cfr in Streptococcus suis. Antimicrob Agents Chemother. 2013;57(8):4061–4063. DOI: 10.1128/AAC.00713-13

Accession number
Contact
Tn6645
Miss Felise Adams
Characteristics
Tn6645 is an 8.5-kb composite transposon, found in Moraxella osloensis CCUG 350 chromosome. It contains two ISAba3 elements, flanking MITEAba12, ISAba11 and ORFs encoding hypothetical protein, alkylsulfatase, TetR regulator, and truncated MFS transporter.
References

Adams FG, Brown MH. MITE Aba12 , a Novel Mobile Miniature Inverted-Repeat Transposable Element Identified in Acinetobacter baumannii ATCC 17978 and Its Prevalence across the Moraxellaceae Family. mSphere. 2019;4(1):e00028-19. Published 2019 Feb 20. DOI: 10.1128/mSphereDirect.00028-19

Accession number
Contact
Tn6646
kaixin zhou
Tn6647
Ricardo León-Sampedro
Characteristics
Tn6647 is a 32.8-kb transposon, found in Enterococcus faecalis strain Ef1. It was predicted to contain 39 ORFs involved in disparate functions that include excision/integration (site-specific integrase), replication, recombination and repair (orf16, a virB4 component), mobilization (Nic relaxase), cell wall biogenesis (PrgA, PrgB, PrgC, PrgT), cell division (chromosome segregation protein and Ftsk) and transcription (HipB, an antitoxin/DNA-binding transcriptional regulator).
References

Ricardo León-Sampedro, Miguel D Fernández-de-Bobadilla, Álvaro San Millán, Fernando Baquero, Teresa M Coque, Transfer dynamics of Tn6648, a composite integrative conjugative element generated by tandem accretion of Tn5801 and Tn6647 in Enterococcus faecalisJournal of Antimicrobial Chemotherapy, , dkz239, DOI: 10.1093/jac/dkz239

Accession number
Contact
Tn6648
Ricardo León-Sampedro
  • Antibiotic Resistance
  • Tetracycline
Characteristics
Tn6648 is a 53.2-kb composite element, found in Enterococcus faecalis strain Ef1. It composes of Tn5801.B15 (∼20.3 kb containing tetM resistance gene) and Tn6647 (∼32.8 kb)
References

Ricardo León-Sampedro, Miguel D Fernández-de-Bobadilla, Álvaro San Millán, Fernando Baquero, Teresa M Coque, Transfer dynamics of Tn6648, a composite integrative conjugative element generated by tandem accretion of Tn5801 and Tn6647 in Enterococcus faecalisJournal of Antimicrobial Chemotherapy, , dkz239, DOI: 10.1093/jac/dkz239

Accession number
Contact
Tn6649
Ms Agila kumari Pragasam
Christian Medical College, Vellore
  • Antibiotic Resistance
  • Amikacin
  • Cephalosporins
  • Gentamicin
  • Imipenem
  • Meropenem
  • Sulphonamide
  • Tetracycline
  • Tobramycin
  • Trimethoprim
  • Antispetic Resistance
  • Quaternary ammonium compound
Characteristics
Tn 6649 is a 31.6 Kb Tn-3 family transposon element, identified in a clinical isolate of extensive drug resistant Pseudomonas aeruginosa. It contains class 1 integrons with multiple classes of antimicrobial resistance genes. This includes 11 AMR genes conferring resistance against beta lactams (blaOXA-10, blaVEB, blaNDM), aminoglycoside (aac(6)II, aadB, aadA1), tetracycline (tetA) and trimethoprim/sulfamethoxazole (dfrB2, sul1).
References

Complete Circular Hybrid genome of ST357 XDR Pseudomonas aeruginosa with dual copies of blaNDM-1 and blaPME in India

Accession number
Contact
Tn6650
Ms Agila kumari Pragasam
Tn6651
Dr. Vincenzo Di Pilato
  • Antibiotic Resistance
  • Erythromycin
Characteristics
Tn6651 is a 3.9-kb composite transposon, found on plasmid pUR-EC07 in Escherichia coli strain EC07. It contains two IS26 elements, flanking ermBL-ermB operon. It shared one of the IS26 with another composite transposon, Tn6652.
References

Di Pilato, V., et al. (2019). "Characterization of the first blaCTX-M-14/ermB-carrying IncI1 plasmid from Latin America." Plasmid 102: 1-5. DOI: 10.1016/j.plasmid.2019.02.001

Accession number
Contact
Tn6652
Dr. Vincenzo Di Pilato
  • Antibiotic Resistance
  • Cephalosporins
Characteristics
Tn6652 is a 10.2-kb composite transposon, found on plasmid pUR-EC07 in Escherichia coli strain EC07. It contains two IS26 elements, flanking ermBL-ermB operon. It shared one of the IS26 with another composite transposon, Tn6652.
References

Di Pilato, V., et al. (2019). "Characterization of the first blaCTX-M-14/ermB-carrying IncI1 plasmid from Latin America." Plasmid 102: 1-5. DOI: 10.1016/j.plasmid.2019.02.001

Accession number
Contact
Tn6653
DanDan Dong
Tn6654
DanDan Dong
Tn6655
David Boyd
National Microbiology Laboratory, Public Health Agency of Canada
  • Antibiotic Resistance
  • Carbapenem
Characteristics
Tn6655 is a 3267 bp composite transposon consisting of two copies of ISAba1 (inversely oriented) flanking 907 bp of DNA that harbours the blaOXA-237 gene (831 bp). It is found in the chromosomes of Acinetobacter baumannii 11A1213CRGN008, 11A1213CRGN055, and 11A14CRGN003 in a single copy and in A. baumannii 11A1314CRGN088 in two copies. It is also found in plasmids pO237-1, pO237-2, pO237-3, and pO237-4 in one, two, two, and one copy, respectively. Note that there can be two different versions of ISAba1 in Tn6655 differing by a nonsynonymous SNP in orf2 (orfB) of the transposase gene such that the mature transposase may have a K204 (AAA) or a Q204 (CAA). Tn6655 is 99% identical to Tn6252 which harbours blaOXA-235.
References

Program, C. N. I. S., et al. (2018). "Results from the Canadian Nosocomial Infection Surveillance Program for detection of carbapenemase-producing Acinetobacter spp. in Canadian hospitals, 2010–16." Journal of Antimicrobial Chemotherapy 74(2): 315-320. DOI: 10.1093/jac/dky416

Accession number
Contact
Tn6656
David Boyd
National Microbiology Laboratory, Public Health Agency of Canada
  • Antibiotic Resistance
  • Carbapenem
Characteristics
Tn6656 is a 3358 bp composite transposon consisting of two copies of ISAba1 (inversely oriented) flanking a 907 bp fragment of DNA that harbours blaOXA-235 (831 bp) with an additional 91 bp of the left end of ISAba1 in inverse orientation found at the right end. Thus, Tn6252 (ISAba1-blaOXA-235-ISAba1; 3267 bp) is wholly contained within Tn6656. Tn6656 is found in three copies in the chromosomes of A. baumannii 09A16CRGN003B and 09A16CRGN014. These two chromosomes also harbour a single copy of Tn6252.
References

Program, C. N. I. S., et al. (2018). "Results from the Canadian Nosocomial Infection Surveillance Program for detection of carbapenemase-producing Acinetobacter spp. in Canadian hospitals, 2010–16." Journal of Antimicrobial Chemotherapy 74(2): 315-320. DOI: 10.1093/jac/dky416

Accession number
Contact
Tn6657
Dr Marco Maria D'Andrea
Tn6658
David Boyd
National Microbiology Laboratory, Public Health Agency of Canada
Characteristics
Tn6658 is a 3940 bp composite transposon consisting of two copies of ISAba1 (inversely oriented) flanking a 1580 bp of DNA that contains a CRP gene (cAMP-activated global transcriptional regulator)(666 bp) and a OsmC family protein gene (osmotically inducible stressed-induced protein)(423 bp). The Tn6658 sequence is found in two copies in the chromosomes of A. baumannii 09A16CRGN014 and 09A16CRGN003B, six copies in 11A1213CRGN008, seven copies in 11A1213CRGN055 and 11A1314CRGN088, and eight copies in 11A14CRGN003. One copy in each chromosome, Tn6658alpha, can be considered to be the progenitor of the transposon as each of the copies of ISAba1 is flanked by their own unique 9 bp direct repeats indicating separate insertion events in this chromosomal region. Tn6658alpha can then be postulated to have transposed as a unit, Tn6658, to a different regions of the chromosome where it inserted by transposition as it is flanked by identical 9 bp direct repeats.
References

Program, C. N. I. S., et al. (2018). "Results from the Canadian Nosocomial Infection Surveillance Program for detection of carbapenemase-producing Acinetobacter spp. in Canadian hospitals, 2010–16." Journal of Antimicrobial Chemotherapy 74(2): 315-320. DOI: 10.1093/jac/dky416

Accession number
Contact
Tn6659
Prof Xiang-Dang Du
Tn6666
Sir Yanpeng Chen

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